STUDIES ON ARGININE

Kinetic studies on the arginine kinase reaction.

A highly purified preparation of arginine kinase from the tail muscle of the crayfish, Panulirus longipes, has been used to investigate the mechanism of the reaction. The results of initial velocity, product inhibition, and isotope exchange at equilibrium studies are consistent with the reaction having a random mechanism in which all the steps are in rapid equilibrium except those concerned wit...

Conformational studies on arginine vasopressin and arginine vasotocin by proton magnetic resonance spectroscopy.

The assignments and partial conformational analysis of the 220-MHz (1)H nuclear magnetic resonance spectrum of the neurohypophyseal hormones arginine vasopressin and arginine vasotocin in uniformaly deuterated dimethylsulfoxide are reported. The chemical shifts, vicinal coupling constants, temperature coefficients of chemical shifts, and proton-deuterium exchange rates for protons of arginine v...

Mechanistic studies on transcriptional coactivator protein arginine methyltransferase 1.

Protein arginine methyltransferases (PRMTs) catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to the guanidinium group of arginine residues in a number of important cell signaling proteins. PRMT1 is the founding member of this family, and its activity appears to be dysregulated in heart disease and cancer. To begin to characterize the catalytic mechanism of this isozyme, we...

Studies on the chemical modification of arginine. I. The reaction of 1,2-cyclohexanedione with arginine and arginyl residues of proteins.

The guanido group of arginine condenses with 1, Z-cyclohexanedione in alkaline aqueous medium to form a new a-amino acid. The product (1 ,2-cyclohexanedione-arginine) was shown by independent synthesis to be N5-(4-0x0-1,3diazaspiro[4.4]non 2 ylidene) L ornithine. At hydroxide concentrations of 0.2 N or higher, this was the principal product, and the reaction with free arginine and with the argi...

Direct inhibition of rat testicular androgen biosynthesis by arginine vasotocin. Studies on mechanisms of action.